# Modelling human adult V-SVZ niche assembly and ependymal cell generation in brain organoids

## TL;DR

This study shows how to generate ependymal cells in brain organoids, mimicking the human adult V-SVZ niche for research.

## Contribution

A novel method using GEMC1 and MCIDAS to program human radial glial cells into ependymal cells in organoids.

## Key findings

- GEMC1 and MCIDAS induce ependymal cell generation in human brain organoids.
- Programmed ependymal cells display human-specific characteristics.
- Ependymal cell generation promotes V-SVZ niche assembly in organoids.

## Abstract

The V-SVZ niche is vital for adult neurogenesis in mammals, yet its regulation in humans remains poorly understood. Current models, including brain organoids, fail to replicate the unique cytoarchitecture of this niche, particularly the multiciliated ependymal cells, which are essential for its function and organization. Here, we utilize GEMC1 and MCIDAS to program human apical radial glial cells into ependymal cells, employing human brain organoids as a model. This approach induces premature ependymal cell differentiation and reorganization of the embryonic neurogenic niche, conferring characteristics of the human adult V-SVZ niche. Our findings highlight a molecular pathway that leads to ependymal cell generation and adult human V-SVZ niche reconstruction, providing a platform to study its development and function.

Human progenitors are programmed into ependymal cells in brain organoids via GEMC1 and MCIDAS expression. This strategy recapitulates hallmarks of the adult human V-SVZ niche, providing a platform to investigate its regulation and function.

GEMC1 and MCIDAS induce human ependymal cell generation.Programmed ependymal cells exhibit intrinsic human-specific characteristics.Ependymal cell generation promotes human adult V-SVZ niche assembly.

GEMC1 and MCIDAS induce human ependymal cell generation.

Programmed ependymal cells exhibit intrinsic human-specific characteristics.

Ependymal cell generation promotes human adult V-SVZ niche assembly.

Human progenitors are programmed into ependymal cells in brain organoids via GEMC1 and MCIDAS expression. This strategy recapitulates hallmarks of the adult human V-SVZ niche, providing a platform to investigate its regulation and function.