# PIONEER: A periplasmic display platform for synthetic biology-based screening of genetically encoded protein regulators

## TL;DR

PIONEER is a new platform for studying protein regulators in yeast, enabling functional screening of membrane proteins and their ligands.

## Contribution

PIONEER introduces a modular periplasmic display system for function-first screening of genetically encoded regulators.

## Key findings

- PIONEER enables detection of surface expression and ligand activity for human G protein-coupled receptors.
- The platform distinguishes intrabodies based on functional effects and chaperone activity.
- PIONEER is adaptable to various membrane and soluble protein targets for scalable functional screening.

## Abstract

Periplasmic display in yeast is a promising but underdeveloped method for screening and studying genetically encoded biomolecules. We present Periplasmic dIsplay Of geNetically Encoded rEgulatoRs (PIONEER), a modular platform that localizes peptides, proteins, and nanobodies to the membrane-proximal periplasmic space of Saccharomycescerevisiae, enabling direct interrogation of membrane protein function. By optimizing secretion signals (ss) and display scaffolds, PIONEER ensures stable ligand retention and robust autocrine signaling. The ability to assess peptide and protein ligand activity through signaling, rather than binding alone, marks a key advance toward function-first screening. Applied to human G protein-coupled receptors, the system enables detection of surface expression, ligand activity profiling, and classification of nanobody regulators, including antagonists and conformational stabilizers. It also distinguishes intrabodies based on their functional effects and chaperone activity. Although demonstrated with G protein-coupled receptors, PIONEER is adaptable to a wide range of membrane and soluble protein targets. As AI-driven design expands the space of candidate ligands and binders, this platform offers a scalable method for linking predicted sequences and their structures to biological function.