# Optimization of somatic embryogenesis protocol for Hevea brasiliensis clones RRIM 600 and REYAN 88-13

## TL;DR

Researchers optimized a method to grow rubber tree embryos in a lab, which could help create genetically modified trees for better rubber production.

## Contribution

The study presents an optimized somatic embryogenesis protocol for two rubber tree clones with contrasting responses.

## Key findings

- Balances in growth regulators are crucial for controlling callus growth and embryogenic capacity.
- The optimized protocol enabled successful genetic transformation trials on two rubber tree clones.
- Callus behavior was analyzed based on embryogenic origin and redox status.

## Abstract

•Optimization of somatic embryogenesis protocol revealed the predominant role of balances in growth regulators.•This optimization should simultaneously control callus growth, tissue browning and embryogenic capacity of cells.•An optimized protocol for somatic embryogenesis facilitates the production of transgenic lines.

Optimization of somatic embryogenesis protocol revealed the predominant role of balances in growth regulators.

This optimization should simultaneously control callus growth, tissue browning and embryogenic capacity of cells.

An optimized protocol for somatic embryogenesis facilitates the production of transgenic lines.

The challenges for the development of rubber cultivation are so agronomically significant in the face of climate change that it is crucial to introduce biotechnology into the clonal propagation of rubber trees. A protocol was developed on rubber clone PB 260. This protocol was used for two other clones, RRIM 600 and REYAN 88-13, which exhibited highly contrasting response to callus growth and embryogenic capacity. Optimization of somatic embryogenesis for these two clones led us to successful conduct genetic transformation trials. We discussed the in vitro behaviour of the calli considering the origin of the embryogenic callus and the endogenous redox status.