# The Complete Mitochondrial Genome of Nearly Threatened Kwang‐Yang Asian Frog Nanorana quadranus (Anura: Dicroglossidae) and Its Phylogenetic Analyses

## TL;DR

This paper reports the first complete mitochondrial genome of a near-threatened frog species from the Qinghai-Tibet Plateau, providing genetic data for its conservation and evolutionary studies.

## Contribution

The first complete mitogenome of Nanorana quadranus is sequenced and analyzed for phylogenetic placement.

## Key findings

- The mitogenome is 20,173 bp long and includes 13 PCGs, 21 tRNAs, 2 rRNAs, and a control region.
- Phylogenetic analysis shows N. quadranus clusters with N. taihangnica within the Dicroglossidae family.
- The study provides foundational genetic data for future conservation and evolutionary research on this species.

## Abstract

Nanorana quadranus
(Liu, Hu & Yang, 1960) is a high‐altitude amphibian species native to the Qinghai‐Tibet Plateau. It plays a vital role in alpine wetland ecosystems and is listed as near threatened (NT) in the IUCN Red List. Here, we present the first complete mitogenome of
N. quadranus
using the Illumina NovaSeq 6000 sequencing platform. The total length of the mitogenome is 20,173 bp (Accession No. PV546862). It encompasses 13 protein‐coding genes (PCGs), 21 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs), and one control region. Phylogenetic analysis revealed that
N. quadranus
forms a clade with
N. taihangnica
, supporting its taxonomic placement within the Dicroglossidae family. This study provides foundational genetic data for further research on the evolution and conservation of this species.

We report the first complete mitochondrial genome of the near‐threatened frog
Nanorana quadranus
from the Qinghai‐Tibet Plateau. The 20,173 bp mitogenome includes 13 protein‐coding genes, 21 tRNAs, 2 rRNAs, and a control region. Phylogenetic analysis places
N. quadranus
in a clade with
N. taihangnica
, providing key genetic data for future studies on evolution and conservation.