# Recent Advances in Lateral Flow Immunoassay for Rapid Diagnosis of Viral Diseases

**Authors:** Quanyu Ren, Yan Wang, Haoyuan Ma, Jialiang Xie, Jianyou Jin, Rumeng Tian, Hao Yu, Xu Gao

PMC · DOI: 10.1155/tbed/5701806 · Transboundary and Emerging Diseases · 2026-01-10

## TL;DR

This paper reviews recent progress in using lateral flow immunoassays for quick and easy detection of viral diseases in humans and animals.

## Contribution

The paper provides a comprehensive overview of LFIA advancements for viral detection, highlighting practical applications and future strategies.

## Key findings

- LFIA is widely used for on-site virus detection due to its speed and portability.
- The review covers technical principles and limitations of LFIA for virus diagnosis.
- Strategies for improving LFIA performance are discussed for better disease control.

## Abstract

Viral diseases are a major threat to human and animal health, as illustrated by recent pandemics like COVID‐19 and African swine fever (ASF). Timely, accurate detection of viral infections is critical for effective disease control. Among diverse diagnostic techniques, lateral flow immunoassay (LFIA) has become a widely used on‐site testing tool, owing to its speed, simplicity, affordability, and portability. The application of LFIA for detecting human and animal viruses is feasible, which highlights its practical utility in veterinary settings. This review summarizes key advances in LFIA for the rapid diagnosis of viral diseases over the past decade, focusing on its technical principles, practical applications, core advantages, existing limitations, and potential effective strategies to provide comprehensive knowledge for virus detection.

## Linked entities

- **Diseases:** COVID-19 (MONDO:0100096), African swine fever (MONDO:0025377)

## Full-text entities

- **Diseases:** ASF (MESH:D000357), Viral Diseases (MESH:D014777), COVID-19 (MESH:D000086382)
- **Species:** Homo sapiens (human, species) [taxon 9606]

## Full text

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## Figures

3 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12790179/full.md

## References

97 references — full list in the complete paper: https://tomesphere.com/paper/PMC12790179/full.md

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Source: https://tomesphere.com/paper/PMC12790179