# Anatomical, Molecular–Genetic, and Phytochemical Study of Species from the Genus Equisetum in Bulgaria

## TL;DR

This study examines five Equisetum species in Bulgaria, analyzing their anatomy, genetics, and chemical composition to explore their potential uses.

## Contribution

The study provides new anatomical, molecular, and phytochemical insights into Equisetum species in Bulgaria.

## Key findings

- Species in subgenus Equisetum have a continuous endodermis ring, while Hippochaete species have a two-layered endodermis.
- DNA barcoding confirmed the taxonomic classification of the studied species.
- Equisetum species showed high sterol and phospholipid content, with palmitic acid and β-sitosterol as major components.

## Abstract

Five species of the genus Equisetum distributed in Bulgaria were studied: four species from the subgenus Equisetum (Equisetum arvense, E. telmateia, E. sylvaticum, and E. palustre) and one from the subgenus Hippochaete (E. ramosissimum). The anatomical, taxonomic, and phylogenetic characteristics of the selected species were established. In species belonging to the subgenus Equisetum, the endodermis was arranged in the form of a continuous ring, while in the representatives of the subgenus Hippochaete, a two-layered endodermis surrounding each vascular bundle was observed. The results from the DNA barcoding supported the taxonomic treatment of the studied species. The chemical and lipid compositions of the plants were also investigated. The Equisetum species had a similar chemical composition and a high content of sterols and phospholipids. In the glyceride oils, palmitic acid predominated, ranging from 69.5% to 78.7%. β-sitosterol was the main component in the sterol fraction, while the tocopherol content was found to be remarkably low in two of the samples (37.6–82.8 mg/kg), with α-tocopherol being predominant. In the phospholipid fraction, the major classes were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, and phosphatidic acids. The chemical composition of the studied species and their high biologically active lipid constituents suggested that they were suitable for application in various directions.