# Progress in Flax Genome Assembly from Nanopore Sequencing Data

## TL;DR

This paper reports high-quality, near-complete genome assemblies for two flax varieties using Nanopore sequencing and Hifiasm, improving on existing flax genome references.

## Contribution

The study presents two telomere-to-telomere quality flax genome assemblies using ONT simplex R10.4.1 data and Hifiasm, without relying on PacBio or ultra-long reads.

## Key findings

- K-3018 and Svyatogor flax genome assemblies reached 491.1 Mb and 497.8 Mb, respectively, with most chromosomes assembled to full length.
- The assemblies surpassed the quality of the current NCBI reference genome for flax variety T397.
- Flax genomes showed high similarity at the chromosome level with only a few large-scale differences.

## Abstract

In recent years, the quality of genome assemblies has notably improved, primarily due to advances in third-generation sequencing technologies and bioinformatics tools. In the present study, we obtained genome assemblies for two flax (Linum usitatissimum L.) varieties, K-3018 and Svyatogor, using Oxford Nanopore Technologies (ONT) simplex R10.4.1 data and the Hifiasm algorithm optimized for ONT reads. The K-3018 genome assembly was 491.1 Mb and consisted of thirteen full-length chromosomes and two one-gap chromosomes. The Svyatogor genome assembly was 497.8 Mb and consisted of twelve full-length chromosomes and three one-gap chromosomes. All chromosomes had telomeric repeats at their ends for both varieties. Hi-C contact maps and Illumina genomic data supported the accuracy of the obtained assemblies. The K-3018 and Svyatogor genome assemblies surpassed the quality of the best currently available flax genome assembly of variety T397, which serves as a reference for L. usitatissimum in the NCBI Genome database. Comparative analysis revealed that the flax genomes are generally quite similar at the chromosome level, with only a few large-scale differences. Thus, two near-T2T (telomere-to-telomere) flax genomes were assembled from the ONT simplex R10.4.1 reads using Hifiasm ONT without involving Pacific Biosciences (PacBio) HiFi or ultra-long ONT reads as well as optical maps. High-quality flax genomes are essential for improving the efficiency of genetic research, evaluating genetic diversity at the whole-genome level, and developing breeding and genome editing approaches of this valuable multipurpose crop.