# In Vitro Antioxidant, Photoprotective, and Volatile Compound Profile of Supercritical CO2 Extracts from Dandelion (Taraxacum officinale L.) Flowers

## TL;DR

This study explores using supercritical CO2 to extract beneficial compounds from dandelion flowers, showing strong antioxidant and sun protection properties.

## Contribution

The study introduces a sustainable SFE-CO2 method with ethanol co-solvent for enhanced extraction of bioactive compounds from dandelion flowers.

## Key findings

- Adding 5% ethanol increased extraction yield by ~50% and improved antioxidant activity significantly.
- The extract showed a high SPF value (49.5), indicating strong photoprotective potential.
- Volatile compounds like carvone and dill ether were identified as major contributors to the extract's aroma.

## Abstract

This study aimed to develop a sustainable approach for isolating bioactive lipophilic components from Taraxacum officinale flowers using supercritical carbon dioxide extraction (SFE-CO2) and to assess the effect of adding 5% ethanol (EtOH) as a co-solvent on extraction yield, in vitro antioxidant capacity in CUPRAC and ABTS assays (TEACCUPRAC and TEACABTS), total phenolic (TPC) and flavonoid (TFC) content, β-carotene concentration, and photoprotective potential, expressed as the sun protection factor (SPF). SFE-CO2 at 35 MPa and 40 °C resulted in 50% of the total yield within 15 min, with equilibrium reached after 120 min (final yield of 4.6 g/100 g flowers). Co-solvent addition increased yield by ~50% and shortened extraction time. The EtOH-modified extract exhibited markedly higher antioxidant activity, with a 2-fold increase in TEACCUPRAC (167 mg TE/g E), an 11-fold increase in TEACABTS (194 mg TE/g E), and a 3-fold increase in TPC (91 mg GAE/g E), along with improved recovery of flavonoids and β-carotene. Volatile profiling revealed monoterpenoids, aldehydes, and esters as dominant groups, with carvone (14.0–16.5%) and dill ether (4.2–5.8%) as major contributors to aroma. The SFE-CO2 + 5% EtOH extract achieved the highest SPF value (49.5 at 1 mg/mL; SPF > 6 at >0.1 mg/mL), indicating strong photoprotective potential and potential suitability for natural antioxidant and cosmetic applications.