# Multifunctional NADES-Based Extracts from Paeonia lactiflora Pall. Flowers for Potential Cosmetic and Pharmaceutical Applications

## TL;DR

This study explores using natural deep eutectic solvents to extract bioactive compounds from peony flowers for use in cosmetics and pharmaceuticals.

## Contribution

The study introduces NADES-based extraction of peony flowers for multifunctional cosmetic and pharmaceutical ingredients.

## Key findings

- NADES extraction yielded high phenolic content with strong antioxidant activity (around 87.5% compared to Trolox).
- Extracts showed antimicrobial activity against multidrug-resistant Staphylococcus and Enterococcus species.
- NADES-based extracts demonstrated anti-biofilm properties and long-term stability of active metabolites.

## Abstract

Paeonia lactiflora Pall. is a perennial herbaceous plant widely renowned for its floral ornamental appeal, distinctive pleasant scent, and utilization in folk remedies. Roots and barks are traditionally used in Chinese medicine for various properties, including anti-inflammatory, antioxidant, antibacterial, anticancer, cardiovascular, and neuroprotective effects. Considering the growing interest and demand in the pharmaceutical and cosmetic fields for sustainable and bioactive botanical derivatives, this study aimed to apply NADES (natural deep eutectic solvents) extraction on fresh flowers of Paeonia lactiflora Pall. The purpose was to obtain a natural, multifunctional, and ready-to-use cosmetic ingredient with concurrent antioxidant activity, antimicrobial functionalities, and olfactive properties. The eutectic systems selected in this study were composed of betaine as the hydrogen bond acceptor and glycerol and/or sorbitol as the hydrogen bond donors. These eutectic systems under microwave activation led to a rapid extraction, from peony fresh flowers, of considerable phenolic amounts (from 33.0 to 34.4 mg of gallic acid equivalents per gram of fresh flowers), which confer to the whole NADES-based extract an excellent radical scavenging activity (around 87.5%, compared to Trolox) and a pleasant fragrance, due to the extraction of some characteristic volatile compounds, as confirmed by GC-MS analysis. Antimicrobial assays against different Gram-positive and Gram-negative strains demonstrated good inhibitory activity of the sample against multidrug-resistant Staphylococcus species (MIC ranging from 0.9 to 14.5 mg/mL) and against Enterococcus species (from 28.8 to 57.8 mg/mL). Furthermore, results on different Staphylococcus aureus strains disclosed additional interesting anti-biofilm properties. Preliminary long-term studies (up to 9 months) on these combined properties highlighted the stabilizing effect of NADESs on the active metabolites, confirming their potential as natural and functional ingredients that could be directly incorporated into pharmaceutical and cosmetic formulations, offering enhanced efficacy and improved stability.