# A New Approach to the Determination of Biogenic Amines in Wine

## TL;DR

This paper introduces a new method for detecting harmful biogenic amines in wine, offering high sensitivity and fast analysis.

## Contribution

A novel derivatization method using TsCl and K2S2O8 for sensitive and rapid detection of biogenic amines in wine.

## Key findings

- The method achieved detection limits as low as 0.00866 mg·L−1 for tyramine in wine.
- Calibration curves showed excellent linearity (r > 0.999) for the analyzed biogenic amines.
- Recovery rates for biogenic amines ranged from 83.0 to 110%, indicating high accuracy.

## Abstract

Biogenic amines (BAs) can be found in various foods, such as cheese, wine, and chocolate. The consumption of a sufficient quantity of BA can lead to symptoms including headaches, hypertonia, and diarrhea. For this reason, the amount of BA in food is regulated in many countries. A new method for the determination of biogenic amines in wine has been proposed, which involves derivatizing BA with p-toluene sulfonyl chloride (TsCl) and using K2S2O8 to reduce the matrix effect. The derivatives of putrescine, cadaverine, histamine, and tyramine with TsCl were synthesized and characterized by 1H NMR spectroscopy. Separation of BA derivatives was performed using a reversed-phase high-performance liquid chromatography (RP-HPLC). The chromatographic system was equipped with a reversed-phase C8 column and a diode array detector. This method was validated to analyze the above-mentioned biogenic amines simultaneously in red and white wine samples. The detection limits for putrescine, cadaverine, histamine, and tyramine in wine samples were 0.0248 mg·L−1, 0.0645 mg·L−1, 0.346 mg·L−1 and 0.00866 mg·L−1, respectively. The calibration curves showed good linearity (r > 0.999), and biogenic amines recovery varied from 83.0 to 110%. The proposed method demonstrates high sensitivity, straightforward sample preparation, and rapid analysis time.