# DNA Origami and Its Applications in Synthetic Biology

## TL;DR

DNA origami is a powerful nanotechnology that allows precise construction of nanostructures, with promising applications in synthetic biology for building artificial life systems.

## Contribution

The paper highlights the integration of DNA origami with cell-free synthetic biology for dynamic regulation and rational design of artificial systems.

## Key findings

- DNA origami enables precise construction and regulation from extracellular to intracellular environments.
- Integration with cell-free systems opens new pathways for artificial life system design.
- The review addresses challenges and future directions in DNA origami-based synthetic biology.

## Abstract

In recent years, DNA origami technology has advanced rapidly as a groundbreaking method for nanomanufacturing. This technology takes advantage of the unique base‐pairing characteristics of DNA, and has significant advantages in constructing spatially ordered and programmable nanostructures. This capability aligns with synthetic biology's core principle of mimicking, extending, and reconstructing natural biological processes by modularly assembling artificial systems. This article provides a comprehensive overview of DNA origami's innovative applications across various domains, including cell membrane surfaces, intercellular communication, intelligent biosensing, and precise gene editing, progressing from the extracellular to the intracellular environment. Finally, this review highlights the synergistic interaction between this technology and cell‐free synthetic biology, achieved through the integration of in vitro assembly and cellular regulation, thereby opening new pathways for the rational design of artificial life systems.

This review provides a comprehensive summary of recent advancements in DNA origami for synthetic biology, emphasizing two key areas: the precise construction and dynamic regulation of extracellular‐to‐intracellular, and the synergistic integration with cell‐free systems. By combining in vitro assembly with cellular regulation, DNA origami synergizes with cell‐free platforms, opening new pathways for innovative approaches in artificial life design. The review also addresses the challenges and future prospects of this field.