# Purification, Structural Characterization, and Immunomodulatory Activities of a Glucan from Morchella sextelata

## TL;DR

This study isolates and characterizes a glucan from Morchella sextelata that shows strong immunomodulatory effects in animal models.

## Contribution

The paper presents a novel structural model and in vivo immunomodulatory evidence for a glucan from Morchella sextelata.

## Key findings

- MSP-1-1 restored immune organ indices and blood parameters disrupted by cyclophosphamide.
- The glucan enhanced macrophage activity, lymphocyte proliferation, and T cell proportions.
- MSP-1-1 increased IgA and IgM levels in serum and thymus, indicating immune system modulation.

## Abstract

In search of natural and safe compounds with immunomodulatory effects, this study identified a glucan with a molecular weight (Mw) of 1.2 × 107 Da, named MSP-1-1, which was extracted and purified from Morchella sextelata via water extraction, alcohol precipitation, and column chromatography. Based on comprehensive characterization using HPAEC, SEC-MALLS-RI, FT-IR, GC-MS, NMR, and SEM, a structural model for MSP-1-1 is proposed. The model depicts a glucan with a backbone predominantly composed of →4)-α-D-Glcp-(1→ linkages, featuring occasional →4,6)-α-D-Glcp-(1→ residues that serve as branch points. The branches are identified as single α-D-Glcp-(1→ units attached at the O-6 position of these branching residues. In vivo experiments revealed that MSP-1-1 restored cyclophosphamide-induced abnormalities in immune organ indices, histology, and peripheral blood parameters. Additionally, MSP-1-1 significantly enhanced macrophage phagocytosis, splenic lymphocyte proliferation, and the proportions of CD3+CD4+ and CD3+CD8+T cells, while increasing the CD3+CD4+/CD3+CD8+ ratio. It also elevated concentrations of IgA and IgM in both serum and thymus, indicating immunomodulatory activity. In summary, this research elucidated the structural characteristics and immunomodulatory activity of MSP-1-1, providing insights into the bioactivity of M. sextelata glucan and a basis for further exploring its potential functional applications.