# Genome-Wide Identification and Analysis of bZIP Transcription Factors in Coptis chinensis Reveals Their Regulatory Roles in Stress Responses

## TL;DR

This study identifies and analyzes bZIP transcription factors in Coptis chinensis, revealing their roles in stress responses and medicinal compound production.

## Contribution

The first genome-wide analysis of bZIP transcription factors in Coptis chinensis, revealing lineage-specific evolution and stress-related gene expression patterns.

## Key findings

- Fifty-five CcbZIP genes were identified and classified into 12 subfamilies, with subfamilies L and O absent.
- Expression of CcbZIP genes was found to respond dynamically to temperature and light stresses in a tissue-specific manner.
- The study provides insights into the regulatory roles of CcbZIPs in stress adaptation and secondary metabolism.

## Abstract

The basic leucine zipper (bZIP) transcription factors play crucial roles in plant growth and stress adaptation. However, a comprehensive genome-wide analysis of this family has been lacking in the medicinal plant Coptis chinensis. In this study, we identified 55 bZIP genes (CcbZIPs) from the C. chinensis genome and systematically classified them into 12 subfamilies through phylogenetic analysis with Arabidopsis thaliana. Notably, subfamilies L and O were absent, while two orphan genes were discovered, indicating lineage-specific evolution. Expression profiling revealed that numerous CcbZIPs respond dynamically to temperature and light stresses in a tissue-specific manner. These findings provide a foundation for understanding the regulatory roles of CcbZIPs in environmental adaptation and secondary metabolism, offering potential genetic targets for future breeding aimed at improving stress tolerance and medicinal compound production in C. chinensis.