# Centrifugation-Assisted Ultrafiltration as an Innovative Methodology to Enhance Phenolic Compound Bioaccessibility and Bioavailability from Winery By-Product Extracts

## TL;DR

A new method called centrifugation-assisted ultrafiltration improves the bioavailability of phenolic compounds from winery by-products.

## Contribution

The study introduces centrifugation-assisted ultrafiltration as a novel technique to enhance the bioaccessibility and bioavailability of phenolic compounds.

## Key findings

- Centrifugation-assisted ultrafiltration removes polymeric proanthocyanidins, increasing low-molecular-weight phenolic compounds.
- PGSE and PSTE showed higher bioaccessibility and bioavailability compared to original extracts.
- Permeates demonstrated greater antioxidant activity and phenolic compound retention after digestion.

## Abstract

An innovative methodology based on a centrifugation-assisted ultrafiltration process (CUF) has been investigated as a suitable methodology to enhance the bioavailability of phenolic compounds with antioxidant activity from winery by-products. For this purpose, seed (GSE) and stem (STE) extracts obtained by pressurized liquid extraction were processed by applying CUF methodology, generating a seed and stem permeate (PGSE and PSTE, respectively). The evaluated methodology allowed for the removal of the polymeric proanthocyanidin fraction. Thus, PGSE and PSTE resulted in a lower number of phenolic compounds and antioxidant activity compared to GSE and STE extracts. However, meanwhile, the low-molecular-weight fraction showed a close trend in its phenolic profile composition, the quantity of the compounds was increased because of a concentration effect in the permeates. Phenolic compounds bioavailability was conducted through an in vitro static digestion method followed by in vitro intestinal absorption using a Caco-2 cell monolayer model. PGSE and PSTE bioaccessibility was greater than STE and GSE because of an intense loss of the polymeric fraction during the digestion process. In addition, higher amounts of total phenolic compounds, as well as low-molecular-weight phenolics, were determined in the PGSE and PSTE bioaccessible fractions. Furthermore, higher antioxidant and total phenolic compounds were detected in the bioavailable fraction after in vitro intestinal absorption assays for the permeates. Hence, CUF methodology resulted as a suitable and effective technique to enhance the phenolic extracts’ bioavailability, although the phenolic matrix effect should be tested.