# Antibacterial Potential and Chemical Composition of Shiitake Mushroom (Lentinus edodes (Berk.) Sing.) Extract Against Pathogenic Bacteria

## TL;DR

This study explores how shiitake mushroom extracts can fight harmful bacteria, identifying which solvents and compounds are most effective.

## Contribution

The study identifies ethyl acetate as the most effective solvent for extracting antibacterial compounds from shiitake mushrooms.

## Key findings

- Ethyl acetate extract showed strongest antibacterial activity against E. coli and S. enterica.
- Ergosterol and linoleic acid were identified as key bioactive compounds with antimicrobial properties.
- Extracts have potential applications in food preservation and pharmaceuticals.

## Abstract

Shiitake mushrooms (Lentinus edodes (Berk.) Sing.) have been widely recognized for their bioactive properties, including antimicrobial activity. This study aimed to investigate the antibacterial potential and chemical composition of shiitake mushroom extracts prepared using different solvents (95% ethanol, ethyl acetate, and chloroform). The highest extraction yield (31.16%) was obtained with 95% ethanol. The antimicrobial activity of the extracts was evaluated using the paper disc diffusion method against nine pathogenic bacterial isolates, including Gram‐positive (Bacillus cereus, Staphylococcus aureus, and S. aureus DMST20654) and Gram‐negative (Escherichia coli, E. coli ATCC25922, Enterobacter cloacae, Pseudomonas aeruginosa, Serratia marcescens, and Salmonella enterica serovar Typhi ATCC16122) bacteria. Plates were incubated at 37°C for 24 h. All experiments were performed in triplicate. The ethyl acetate extract exhibited the strongest antibacterial activity, with the largest inhibition zone observed for E. coli ATCC25922 (30.00 ± 0.00 mm), followed by S. enterica serovar Typhi ATCC16122 (28.33 ± 2.00 mm). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays confirmed the superior antibacterial potential of the ethyl acetate extract, particularly against S. aureus DMST20654 (MIC = 1.95 mg/mL, MBC = 31.25 mg/mL). Gas chromatography–mass spectrometry (GC–MS) analysis identified key bioactive compounds, including ergosterol (62.38%, %Prob 62.6 in the chloroform extract) and linoleic acid (28.65%, %Prob 56.5 in the ethyl acetate extract), which are known for their antimicrobial properties. The findings highlight the potential of ethyl acetate‐extracted shiitake mushroom compounds as natural antibacterial agents, with applications for food preservation, nutraceuticals, and pharmaceuticals.