# LncRNA FEZF1-AS1 regulates the gene expression and alternative splicing associated with osteosarcoma

## TL;DR

This study shows that the lncRNA FEZF1-AS1 promotes osteosarcoma cell growth and invasion by regulating gene expression and splicing.

## Contribution

The study identifies FEZF1-AS1 as a novel regulator of gene expression and alternative splicing in osteosarcoma.

## Key findings

- FEZF1-AS1 overexpression increases HOS cell proliferation and invasion.
- FEZF1-AS1 upregulates 125 genes and suppresses 48 genes in osteosarcoma cells.
- FEZF1-AS1 impacts 1,156 alternative splicing events, including pathways like C-type lectin receptor signaling.

## Abstract

Osteosarcoma (OS) faces a high metastatic burden, limited effective treatments, and a lack of specific therapeutic targets.

In this prospective study, we established a human osteosarcoma cell (HOS) model overexpressing lncRNA FEZF1-AS1 (OE FEZF1-AS1), and subsequently obtained FEZF1-AS1-affected transcriptome data using high-throughput RNA sequencing (RNA-seq).

OE FEZF1-AS1 promoted HOS cell proliferation and invasion, upregulated 125 genes, suppressed 48 genes, and impacted 1156 alternative splicing events (ASEs), including pathways like C-type lectin receptor signaling and Yersinia pestis infection.

LncRNA FEZF1-AS1 plays a pivotal role in promoting the proliferation and invasion of HOS cells. Moreover, our study provides a comprehensive gene screening approach for identifying potential targets of FEZF1-AS1, emphasizing their regulatory mechanisms and ASEs in OS.

The online version contains supplementary material available at 10.1186/s13018-025-06357-z.