Physical stimuli-responsive CRISPR-Cas9 systems for spatiotemporally precise control of genome engineering
Jinbin Pan, Bingjie Li, Yaqiong Wang, Yating Han, Guijun Liu, Shao-Kai Sun
TL;DR
This paper reviews how physical stimuli can control CRISPR-Cas9 to improve precise genome editing in specific cells or tissues.
Contribution
The paper introduces physical stimuli-responsive CRISPR-Cas9 systems as a novel approach for spatiotemporal control of genome editing.
Findings
Physical stimuli offer superior spatiotemporal precision and biocompatibility compared to chemical inducers.
Current physical stimuli-responsive systems show improved controllability and target specificity.
A comparative analysis highlights limitations and future directions for clinical translation.
Abstract
The Clustered Regularly Interspaced Short Palindromic Repeats-associated protein 9 (CRISPR-Cas9) endonuclease system has revolutionized biology research by enabling precise, efficient, and versatile genome editing. However, achieving spatiotemporally controlled gene editing within specific organs, tissues, or cells remains a major challenge, as unregulated CRISPR-Cas9 activity can lead to severe off-target effects, hindering its clinical translation. To enhance the on-target precision and reduce the unwanted consequences of aberrant or premature CRISPR-Cas9 activation, various strategies have been developed to regulate its function at translational or post-translational stages using diverse external physicochemical stimuli. While chemical molecule-inducible CRISPR-Cas9 systems have demonstrated significant progress, most of them still suffer from inherent deficiencies, such as…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
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Taxonomy
TopicsCRISPR and Genetic Engineering · Advanced biosensing and bioanalysis techniques · Pluripotent Stem Cells Research