# Extraction process and antioxidant and antimicrobial activities of total flavonoids from Broussonetia papyrifera leaves

## TL;DR

This study developed an efficient method to extract flavonoids from Broussonetia papyrifera leaves, which showed strong antioxidant and antimicrobial properties.

## Contribution

The study introduces an optimized extraction process for flavonoids with proven antioxidant and antimicrobial activities.

## Key findings

- The optimized flavonoids effectively scavenged DPPH, hydroxyl, and ABTS radicals.
- The extract showed potent antibacterial activity against S. aureus, E. coli, and C. albicans.
- UPLC-MS/MS identified numerous flavonoid compounds in the extract.

## Abstract

This study developed an efficient extraction process for flavonoids from Broussonetia papyrifera leaves using the response surface methodology. The optimized flavonoids exhibited remarkable antioxidant activity, effectively scavenging DPPH, hydroxyl, and ABTS radicals. UPLC-MS/MS analysis preliminarily identified numerous flavonoid compounds in the extract.. The extract also demonstrated potent broad-spectrum antibacterial efficacy against Staphylococcus aureus, Escherichia coli, and Candida albicans. These findings indicate that the extracted flavonoids may serve as natural antioxidants and antimicrobials in the food, pharmaceuticals, and cosmetics industries.