# Comparative Analysis of Vehicles for the Regeneration of Mouse Endometrial Damage Model

## TL;DR

This study compares two methods for repairing endometrial damage in mice and finds that a patch-based approach works better than direct cell injection.

## Contribution

The study introduces a novel SIS-based EMSC patch for endometrial regeneration and demonstrates its superiority over direct cell injection.

## Key findings

- The SIS-based EMSC patch significantly improved endometrial thickness compared to EMSC injection.
- The patch group showed better gland formation and fibrosis reduction than the injection group.
- Scaffold-assisted cell therapy is proposed as a promising treatment for endometrial damage-related infertility.

## Abstract

Endometrial damage is a critical factor contributing to infertility, particularly in women with refractory thin endometrium or intrauterine adhesions. Therefore, developing innovative therapeutic strategies for endometrial regeneration is essential. This study evaluates the regenerative potential of endometrial stromal cell (EMSC) injection and EMSC-loaded patch application in a mouse model with ethanol-induced endometrial damage.

A mouse model of endometrial damage was established using ethanol injection into the uterine horn. EMSCs were isolated, cultured, and either HA-injected into the damaged endometrium or transplanted via a small intestinal submucosa (SIS)-based EMSC patch. Histological analyses were performed to assess endometrial thickness, gland regeneration, and fibrosis reduction.

Both EMSC injection and SIS-based EMSC patch engraftment promoted endometrial regeneration. However, the SIS-based EMSC patch group exhibited significant improvements in endometrial thickness, gland formation, and fibrosis reduction compared to the EMSC injection group.

This study demonstrates the superior regenerative potential of an SIS-based EMSC patch over direct EMSC injection for endometrial repair. The findings suggest that scaffold-assisted cell therapy could be a promising approach for treating endometrial damage-related infertility. Further studies are required to optimize this strategy for clinical applications.