# SOSHI-seq: a high-throughput screening assay to test the functionality of putative response elements for nuclear hormone receptors

## TL;DR

SOSHI-seq is a new, fast, and cost-effective method to test how well DNA sequences function as hormone-response elements for nuclear receptors.

## Contribution

SOSHI-seq adapts STARR-seq to screen thousands of DNA sequences for hormone-response functionality in a high-throughput manner.

## Key findings

- SOSHI-seq effectively identifies functional hormone-response elements for nuclear receptors like thyroid hormone receptors.
- The method complements ChIP-Seq by enabling genome-wide screening of response elements in chromatin.
- SOSHI-seq is versatile, rapid, and suitable for large-scale functional genomics studies.

## Abstract

We present a cheap, rapid, and versatile assay called SOSHI-seq (Screening of Self-transcribed Hormone Inducible response elements coupled to sequencing). The protocol is an adaptation of the STARR-seq method, which allows testing the capacity of thousands of synthetic DNA sequences to act as hormone-response elements. Using the nuclear receptors of thyroid hormone as an example, we show that SOSHI-seq is a suitable complement to ChIP-Seq analysis to identify at genome-wide scale the functional response elements occupied by nuclear receptors in chromatin.

The online version contains supplementary material available at 10.1038/s41598-025-29970-8.