# De novo genome assembly of Ansell's mole-rat (Fukomys anselli)

**Authors:** Milica Bekavac, Raphael Coimbra, Veronica F Busa, Mikaela Behm, Rebecca E Wagner, Angela Goncalves, Sabine Begall, Michaela Frye, Duncan T Odom

PMC · DOI: 10.1093/g3journal/jkaf271 · G3: Genes | Genomes | Genetics · 2025-11-11

## TL;DR

This paper presents the first high-quality genome assembly of Ansell's mole-rat, enabling future studies on its unique traits and evolution.

## Contribution

The novel contribution is the first chromosome-level genome assembly of Ansell's mole-rat using a hybrid sequencing approach.

## Key findings

- A chromosome-level genome assembly of 2.27 Gb with 412 scaffolds and 72.4 Mb scaffold N50 was generated.
- 99.54% of expected genes were identified, with 29,094 transcripts annotated using RNA sequencing data.

## Abstract

Ansell's mole-rat (Fukomys anselli) is an African rodent known for its subterranean lifestyle and unique phenotypic traits, including extreme longevity, magnetoreception, and a cooperative breeding social structure. Efforts to dissect the genetic architecture of these traits and to decipher their phylogenetic relationships within the broader African mole-rat family would greatly benefit from a reference-grade genome. Here, we report a first genome assembly of a male Ansell's mole-rat. By combining Oxford Nanopore Technologies long reads and Illumina short reads with Hi-C data, we generated a chromosome level assembly with a total length of 2.27 Gb, 412 scaffolds, and a scaffold N50 of 72.4 Mb. We identified 99.54% of expected genes and annotated 29,094 transcripts using RNA sequencing data. This high-quality de novo genome of F. anselli lays the foundation for dissecting the genetic and evolutionary basis of its extraordinary traits and resolving African mole-rat phylogeny.

## Linked entities

- **Species:** Fukomys anselli (taxon 261002)

## Full-text entities

- **Species:** Rodentia (rodent, order) [taxon 9989], Cryptomys hottentotus (African mole rat, species) [taxon 10175], Fukomys anselli (Ansell's mole-rat, species) [taxon 261002]

## Full text

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## Figures

3 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12774600/full.md

## References

77 references — full list in the complete paper: https://tomesphere.com/paper/PMC12774600/full.md

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Source: https://tomesphere.com/paper/PMC12774600