# Evaluation of Safety and Antigenotoxic Activity of Rosa centifolia Extract, Kaempferol, and Kaempferol-3-glucoside against Ultraviolet B Radiation in Human Fibroblasts

## TL;DR

This study evaluates the safety and DNA-protecting effects of Rosa centifolia extract and flavonoids against UVB radiation in human cells.

## Contribution

The study identifies Rosa centifolia extract and specific flavonoids as potential photoprotective agents against UVB-induced DNA damage.

## Key findings

- R. centifolia extract, kaempferol, and kaempferol-3-glucoside reduced UVB-induced DNA damage in human fibroblasts at noncytotoxic concentrations.
- Quercetin and R. centifolia extract showed genotoxic effects at high concentrations.
- The compounds represent promising candidates for sunscreen formulations.

## Abstract

The plants can be
a source of compounds that prevent UV-induced
DNA damage involved in the genesis of skin cancer and aging. This
work was aimed to evaluated the safety and the antigenotoxic effect
of Rosa centifolia flower ethanolic
extract and of selected flavonoid constituents against UVB radiation
in MRC-5 human fibroblasts. The cytotoxicity and genotoxicity of the
phytochemicals were evaluated using trypan blue exclusion and Comet
assays, respectively. The assays revealed that R. centifolia extract, kaempferol, kaempferol-3-glucoside, and quercetin exhibited
cytotoxic effects at concentrations of 363 μg/mL, 393 μM,
379 μM, and 141.1 μM, respectively. Additionally, R. centifolia extract and quercetin demonstrated
genotoxic effects at the highest tested concentrations. The antigenotoxic
effects of R. centifolia extract, kaempferol,
and kaempferol-3-glucoside against UVB radiation were subsequently
evaluated. These phytochemicals significantly reduced UVB-induced
DNA damage in human fibroblasts at noncytotoxic concentrations. Therefore,
these compounds represent promising candidates for sunscreen formulations
for human photoprotection.