# Lipidomic Comparison of Detergent‐Soluble and Detergent‐Resistant Lipid Domains Isolated From Rat Cerebellum and the Effect of Hypoxia–Ischemia Using UPLC‐HDMSE

## TL;DR

This study uses lipidomics to compare changes in brain membrane lipids after hypoxia-ischemia in rat cerebellum, focusing on detergent-resistant and detergent-soluble domains.

## Contribution

The study introduces untargeted lipidomics to analyze specific lipid changes in membrane domains after hypoxia-ischemia in a rat model.

## Key findings

- Lipid changes in detergent-resistant domains included lysophospholipids, sphingomyelins, and ceramides linked to neuronal impairment.
- Altered lipid composition in membrane domains may explain structural and functional disruptions after hypoxia-ischemia.
- Advanced lipidomics tools enabled detailed analysis of lipid species in distinct membrane fractions.

## Abstract

Hypoxic ischemic encephalopathy (HIE) due to insufficient oxygen or blood flow to the brain can result in inflammation, impaired neurodevelopment, or death. Changes to microdomain lipid composition may contribute to altered structure and function of key regulatory processes; however, knowledge regarding changes to membrane lipid composition after HIE is incomplete. Here, we describe the application of untargeted lipidomics to investigate the impact of hypoxia–ischemia on detergent‐resistant membrane (DRM) and detergent‐soluble membrane (DSM) domains fractionated from the cerebellum in a rat model of HIE. Lipidomics utilized ultra performance liquid chromatography coupled to data independent tandem mass spectrometry with traveling wave ion mobility. Lipid alterations specific to the DRM domains after hypoxia–ischemia included lysophospholipids, sphingomyelins, ceramides, triglycerides, and phosphatidylethanolamines, which are lipid species that have been linked to cognitive and neuronal impairment. The advances in lipidomics have enabled the tools available to study lipid composition. These data may provide insight into membrane disruption after HIE in relation to lipid composition and concentration.