# The HLA landscape of Colombia: a high-resolution analysis of 11,576 blood donors

## TL;DR

This study maps the diverse HLA genes in Colombia's population using data from thousands of blood donors, revealing insights important for transplantation and genomic medicine.

## Contribution

The first large-scale, high-resolution HLA analysis of Colombia's admixed population, capturing extensive immunogenetic diversity.

## Key findings

- Identified 565 HLA alleles and 17,317 unique haplotypes in Colombian blood donors.
- Strong linkage disequilibrium between HLA class I and II loci suggests conserved extended haplotypes.
- Colombian populations cluster closely with Indigenous Chilean groups, indicating shared Andean ancestry.

## Abstract

The highly polymorphic Human Leukocyte Antigen (HLA) system is critical for adaptive immunity and determines compatibility in transfusion and transplantation medicine. Admixed Latin American populations, such as Colombia’s trihybrid population, possess unique HLA diversity that remains poorly characterized at high resolution.

We performed high-resolution HLA typing on 11,576 Colombian blood donors from two distinct regions: the Andean and Caribbean. We analyzed allele and haplotype frequencies, Hardy-Weinberg equilibrium, linkage disequilibrium (LD), and compared genetic distances with global populations via PCA and UPGMA clustering.

We identified 565 alleles and 17,317 unique haplotypes, revealing extreme diversity. A few alleles dominated each locus, yet the top 20 haplotypes had a cumulative carrier frequency of only 3.17%, highlighting a fragmented haplotype landscape. Strong LD was observed between class I and II loci (HLA-A~DQB1), indicating conserved extended haplotypes. Genetically, the Colombian cohorts formed a tight cluster, showing closest affinity to Indigenous Chilean populations, suggesting a shared Andean background.

This study provides the first large-scale, high-resolution HLA map of Colombia, capturing the extensive immunogenetic diversity of an admixed Latin American population. Our findings are vital for improving transplant matching, understanding population-specific disease risks, and advancing equitable genomic medicine in the region.