# Human tolerogenic dendritic cell subtypes exert divergent effects on induction of cytotoxic CD4+ T cells

## TL;DR

This study compares different types of tolerogenic dendritic cells and finds that some can induce cytotoxic activity in CD4+ T cells, which could impact their use in treating autoimmune diseases.

## Contribution

The study identifies that vitamin D3-generated tolerogenic dendritic cells uniquely induce cytotoxic CD4+ T cells, a novel regulatory mechanism.

## Key findings

- Tolerogenic dendritic cells generated with vitamin D3 induce cytotoxic CD4+ T cells marked by granzyme B and cytotoxic gene expression.
- TolDC produced with vitamin D3 and dexamethasone do not induce cytotoxic activity in CD4+ T cells.
- Both tolDC types show reduced interleukin-12 production and interferon-γ secretion by CD4+ T cells.

## Abstract

Tolerogenic dendritic cells (tolDC) are currently in clinical trials for the treatment of autoimmune diseases such as rheumatoid arthritis and multiple sclerosis. The methods for producing therapeutic tolDC vary widely, with little being known about the commonalities and differences of distinct cell products in terms of their regulatory actions on CD4+ T cells. We compared human monocyte-derived tolDC generated with vitamin D3 alone or in combination with dexamethasone. We found marked differences in the surface expression of HLA-DR and immune regulatory molecules, but also found commonalities, e.g. a strongly reduced capacity to produce interleukin-12 and a concomitant decreased ability to induce interferon-γ secretion by allogeneic CD4+ T cells. To gain a deeper understanding of how these tolDC types exert their regulatory effects, we co-cultured them with CD4+ T cells from rheumatoid arthritis patients or healthy controls and analysed the gene expression profile and function of the responding T cells. We found that tolDC generated with vitamin D3 alone, but not in combination with dexamethasone, induced potent cytotoxic activity in the responding CD4+ T cells as demonstrated by an enhanced cytotoxic gene signature, increased levels of intracellular granzyme B, and superior cytotoxic activity towards myeloid and B cells. These data identify cytotoxicity as an atypical CD4+ T helper cell effector function induced by some but not all tolDC types, with implications for their individual clinical applications.