# Mammalian synthetic gene circuits for biopharmaceutical development & manufacture

## TL;DR

This paper reviews how synthetic gene circuits in mammals can be used to improve the production of biopharmaceuticals like antibodies and gene therapies.

## Contribution

The paper provides a comprehensive review of design principles and applications of synthetic gene circuits in mammalian systems for biopharmaceuticals.

## Key findings

- Synthetic gene circuits can regulate monoclonal antibody glycosylation for better therapeutic outcomes.
- Computational modeling helps optimize gene circuits for biopharmaceutical production.
- Gene circuits can enhance safety in CAR-T cell therapy and increase viral vector yields in gene therapy.

## Abstract

This paper reviews the design and application of mammalian synthetic gene circuits for biopharmaceutical manufacturing. It discusses key design principles and outlines transcription factors, DNA-binding proteins, and RNA as input and regulatory modules, while also presenting computational modelling as a driver for circuit optimisation. The review highlights potential applications towards the production of next-generation biotherapeutics by providing examples on monoclonal antibody glycosylation control, CAR-T cell therapy safety, and gene therapy viral vector yields.