# LINC01121 Is Associated With Prognosis and Facilitates the Proliferation, Migration, and Invasion of Colorectal Cancer

## TL;DR

LINC01121 is highly expressed in colorectal cancer and linked to worse outcomes, suggesting it could be a useful diagnostic and prognostic marker.

## Contribution

This study identifies LINC01121 as a novel prognostic biomarker and oncogenic driver in colorectal cancer.

## Key findings

- LINC01121 is overexpressed in CRC tissues compared to adjacent normal tissues.
- High LINC01121 expression correlates with poor survival and immune suppression in CRC patients.
- LINC01121 promotes CRC cell proliferation, migration, and invasion in vitro.

## Abstract

The role of LINC01121 in the pathogenesis and prognosis of colorectal cancer (CRC) remains unknown. This study aimed to explore the function of LINC01121 in CRC development.

Transcriptome expression data from The Cancer Genome Atlas (TCGA) database were utilized to investigate the relationship between LINC01121 and CRC prognosis through survival analysis. Samples from CRC patients and adjacent tissues were collected and analyzed to assess the expression differences between tumor and adjacent tissues. Functional assays such as Cell Counting Kit-8 (CCK8), EdU, scratch test, and Transwell assay were employed to determine the oncogenic role of LINC01121 in CRC cells. Additionally, gene enrichment analyses and immune infiltration analyses were conducted with R package.

The LINC01121 level was obviously higher in CRC tissues. The receiver operating characteristic (ROC) curve suggests that LINC01121 has significant diagnostic capabilities (area under the curve (AUC) = 0.659). High expression of LINC01121 predicted poor overall survival (OS) (P = 0.022), progression-free interval (PFI) (P = 0.007), and disease-specific survival (DSS) (P = 0.006). Gene Set Enrichment Analysis (GSEA) demonstrated that LINC01121-associated CRC encompasses various crucial pathways linked to tumorigenesis. The immune infiltration analyses revealed that LINC01121 may be involved in immune suppression. In vitro experiments demonstrated that LINC01121 facilitated the proliferation, migration, and invasion of CRC cells.

LINC01121 exhibits elevated expression in CRC and correlates with unfavorable prognosis and reduced immune infiltration in CRC patients. These findings suggest that LINC01121 may serve as a potential marker for the diagnosis and prognosis of CRC.