# Legacy and impact of the 1925 Geneva Protocol: one hundred years of treaties and debates on chemical and biological weapons

**Authors:** Walter E. Grunden, Olli H. Tuovinen

PMC · DOI: 10.3389/fmicb.2025.1685967 · Frontiers in Microbiology · 2025-12-17

## TL;DR

This essay reviews the 1925 Geneva Protocol's legacy in banning chemical and biological weapons, highlighting its successes and shortcomings over the past century.

## Contribution

The essay provides a historical analysis of the Geneva Protocol and its successor treaties, emphasizing their mixed legacy and the factors influencing compliance.

## Key findings

- The 1925 Geneva Protocol was a milestone in banning chemical and biological weapons but lacked provisions for verification and enforcement.
- Despite the protocol, limited use of these weapons occurred, suggesting other factors like political deterrence and technological limitations played a role.
- The essay highlights the need for reflection on the efficacy of these treaties and their relevance in modern contexts.

## Abstract

This essay examines the legacy and impact of the 1925 Geneva Protocol, which prohibited the use of chemical and biological weapons. This multinational treaty was an important milestone in the history of the non-proliferation of weapons of mass destruction concluded in the wake of the horror engendered by the use of poison gases in World War I. However, the 1925 protocol did not address the issues of verification, manufacturing, stockpiling, and transferring products of chemical and biological weapons or production technologies. A second treaty, the result of the Biological Weapons Convention of 1972, was concluded to address these issues. Despite the apparent success of prohibiting large-scale and widespread use of chemical and biological weapons, violations have occurred, nonetheless, and the treaties remain problematic in numerous ways. The centennial of this historic 1925 agreement and its successor treaties presents an opportunity for reflection upon their efficacy. Given that there have been “limited” episodes of chemical and biological warfare since that time, how can these events be explained in light of the protocol’s prohibitions? This essay argues that the 1925 Geneva Protocol has a mixed legacy and, at best, can be deemed only a qualified success. Other factors, such as political deterrence, scientific and technological limitations, and the problematic nature of these types of weapons may account for the absence of their mass use. The essay aims to serve as an introduction to the historiographical literature with an emphasis on biological weapons and the Geneva Protocol and includes tables as reference guides concerning the types of weaponized pathogens and toxins in question.

## Full-text entities

- **Diseases:** Zoonotic diseases (MESH:D015047), glanders (MESH:D005896), bacterial blight (MESH:D001424), acute radiation syndrome (MESH:D054508), botulism (MESH:D001906), cholera (MESH:D002771), dengue (MESH:D003715), plague (MESH:D010930), common rust (MESH:D020326), hemorrhagic diseases (MESH:D006470), Ebola (MESH:D019142), crop damage (MESH:D020263), animal and plant diseases (MESH:D000820), red rust (MESH:C562718), SARS (MESH:D045169), yellow fever (MESH:D015004), brucellosis (MESH:D002006), Lassa (MESH:D007835), Infectious Diseases (MESH:D003141), tularemia (MESH:D014406), malaria (MESH:D008288), Newcastle poultry disease (MESH:D009521), Venezuelan equine encephalitis (MESH:D004685), black stem rust (MESH:D007898), typhoid (MESH:D014435), psittacosis (MESH:D009956), smallpox (MESH:D012899), panic (MESH:D016584), viral hemorrhagic fever (MESH:D006482), Bolivian hemorrhagic fever (MESH:D006478), influenza (MESH:D007251), death (MESH:D003643), Marburg (MESH:D008379), tobacco blue mold disease (MESH:D014029), typhus, paratyphus, (MESH:D014438), dysentery (MESH:D004403), melioidosis (MESH:D008554), Anthrax (MESH:D000881), Foot-and-mouth (MESH:D005536), meningitis (MESH:D008580), infection (MESH:D007239), white mold (MESH:D000090122), Stem rust (MESH:D020295), post (MESH:D000094025), poisoning (MESH:D011041), COVID-19 (MESH:D000086382), fatalities (MESH:C565541), Cancer (MESH:D009369)
- **Chemicals:** hydrogen cyanide (MESH:D006856), VX (MESH:C009680), phosgene (MESH:D010705), aflatoxins (MESH:D000348), CS (MESH:D002586), Agent Orange (MESH:D000075182), formaldehyde (MESH:D005557), 210Po (MESH:C000615141), SO2 (MESH:D013458), quinine (MESH:D011803), methylmercury chloride (MESH:C004925), glutaraldehyde (MESH:D005976), tetrodotoxin (MESH:D013779), hydrogen (MESH:D006859), ammonia (MESH:D000641), CS tear (-), sarin (MESH:D012524), mustard gas (MESH:D009151), 2,3,7,8-tetrachlorodibenzo-p-dioxin (MESH:D000072317), tabun (MESH:C009374), Chlorine (MESH:D002713), potassium permanganate (MESH:D011196), peracetic acid (MESH:D010463), nitrogen (MESH:D009584), dodecylamine (MESH:C003935)
- **Species:** Coxiella burnetii (species) [taxon 777], Bacillus anthracis (anthrax bacterium, species) [taxon 1392], Ovis aries (domestic sheep, species) [taxon 9940], Serratia marcescens (species) [taxon 615], Severe acute respiratory syndrome coronavirus 2 (no rank) [taxon 2697049], Bos taurus (bovine, species) [taxon 9913], Equus caballus (domestic horse, species) [taxon 9796], Variola virus (smallpox virus, no rank) [taxon 10255], Ustilago hordei (species) [taxon 120017], Francisella tularensis (species) [taxon 263], Puccinia helianthi (species) [taxon 652029], Solanum tuberosum (potatoes, species) [taxon 4113], Dengue virus (no rank) [taxon 12637], Bacillus licheniformis (species) [taxon 1402], Puccinia graminis f. sp. tritici (forma specialis) [taxon 56615], Brassica oleracea (wild cabbage, species) [taxon 3712], Orthopoxvirus vaccinia (species) [taxon 10245], Capra hircus (domestic goat, species) [taxon 9925], Bacillus sp. AT (species) [taxon 1196779], Francisella tularensis subsp. tularensis (subspecies) [taxon 119856], Sclerotinia sclerotiorum (species) [taxon 5180], Tobacco mosaic virus (no rank) [taxon 12242], Bubalus bubalis (domestic water buffalo, species) [taxon 89462], Allium cepa (onion, species) [taxon 4679], Ricinus communis (castor bean, species) [taxon 3988], Monkeypox virus (no rank) [taxon 10244], Camelpox virus (no rank) [taxon 28873], Sus scrofa (pig, species) [taxon 9823], Yellow fever virus (no rank) [taxon 11089], Phytophthora infestans (potato late blight agent, species) [taxon 4787], Ebola virus (no rank) [taxon 1570291], Bacillus subtilis (species) [taxon 1423], Cucumber mosaic virus (cucumber mosaic cucumovirus, no rank) [taxon 12305], Daucus carota (carrot, species) [taxon 4039], Viruses (acellular root) [taxon 10239], Bacillus atrophaeus (species) [taxon 1452], Clostridium perfringens (species) [taxon 1502], Thrips palmi (species) [taxon 161013], Puccinia graminis (wheat stem rust, species) [taxon 5297], Pseudomonas savastanoi (species) [taxon 29438], Homo sapiens (human, species) [taxon 9606], tomato spotted wilt virus [taxon 1933298], Escherichia coli O157:H7 (no rank) [taxon 83334], Tomato yellow leaf curl virus (no rank) [taxon 10832], Leptinotarsa decemlineata (Colorado potato beetle, species) [taxon 7539], Burkholderia mallei (species) [taxon 13373], Mus musculus (house mouse, species) [taxon 10090], Clostridium botulinum (species) [taxon 1491], Bacteria Latreille et al. 1825 (Bacteria stick insect, genus) [taxon 629395]

## Full text

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## References

111 references — full list in the complete paper: https://tomesphere.com/paper/PMC12755154/full.md

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Source: https://tomesphere.com/paper/PMC12755154