# Triple rAAV9 Vector Combinations Encoding Broadly Neutralizing Antibodies Effectively Suppress HIV-1 Infection in Humanized Mice

## TL;DR

This study shows that using combinations of three rAAV9 vectors encoding antibodies can effectively prevent HIV-1 infection in humanized mice.

## Contribution

The novel contribution is demonstrating the protective efficacy of triple rAAV9 vector combinations against HIV-1 in humanized mice.

## Key findings

- Mice treated with CombiMab-1 or CombiMab-2 did not develop viremia after HIV-1 challenge.
- Human CD4+ T-lymphocyte counts were maintained in treated mice.
- The results highlight the protective capacity of these vector combinations against HIV-1.

## Abstract

This study investigated the protective efficacy of two distinct combinations of three recombinant adeno-associated virus serotype 9 (rAAV9) vectors encoding broadly neutralizing antibodies against HIV-1—CombiMab-1 and CombiMab-2—in mice humanized with primary CD4+ T-lymphocytes. We demonstrated that mice preventively treated with CombiMab-1 or CombiMab-2 did not develop viremia and maintained human CD4+ T-lymphocyte counts following viral challenge, in contrast to control animals. These results demonstrate the significant protective capacity of CombiMab-1 and CombiMab-2 against HIV-1 challenge.