# Comparison of Alcohol-Induced Hepatoprotective Effects of the High Fischer Ratio Oligopeptides with/Without Half Substitution by Pueraria lobata

## TL;DR

This study investigates how combining high Fischer ratio oligopeptides with Pueraria lobata extract protects the liver from alcohol damage in cells and mice.

## Contribution

The novel contribution is demonstrating the enhanced hepatoprotective effects of combining HFOPs with Pueraria lobata extract.

## Key findings

- HFOPs + PL reduced ALT and AST leakage and improved antioxidant activity in alcohol-induced HepG2 cells.
- The combination prolonged intoxication time and improved ethanol metabolism in mice.
- HFOPs + PL showed better liver protection than PL alone and comparable effects to HFOPs alone.

## Abstract

Long-term excessive intake of alcohol can cause serious damage to the liver, and the study of natural active ingredients with hepatoprotective effects is of great significance for the prevention and treatment of alcoholic liver injury. This study explored the ameliorative effects of high F-value oligopeptides (HFOPs) from Chlamys farreri, Pueraria lobata extract (PL), and their complex (HFOPs + PL) on alcoholic liver injury. Results showed HFOPs + PL significantly reduced alanine aminotransferase (ALT) and aspartate aminotransferase (AST) leakage, increased superoxide dismutase (SOD) and glutathione (GSH) activities, and decreased the production of malondialdehyde (MDA) and inflammatory factors in alcohol-induced HepG2 cells. In mice, it prolonged intoxication time, shortened detoxification time, enhanced hepatic ADH and aldehyde dehydrogenase (ALDH) activities, reduced serum AST and ALT levels, and improved antioxidant capacity. Its effects were better than PL alone and comparable to HFOPs alone. HFOPs and PL alleviate alcoholic liver injury by enhancing ethanol metabolism, reducing oxidative stress, and suppressing inflammation, providing theoretical support for their combined use in alcohol detoxification and liver protection.