# Characteristics, Domestication and Nutrient Analysis of Wild Pleurotus placentodes

## TL;DR

This study explores a wild mushroom species from Tibet, identifies it as Pleurotus placentodes, and determines its optimal growth and nutritional properties for potential industrial use.

## Contribution

The study provides new insights into the domestication and nutritional profile of wild Pleurotus placentodes for industrial cultivation.

## Key findings

- Pleurotus placentodes thrives on fructose/sucrose, yeast/beef powder, pH 5.0, and 20°C.
- The mushroom completes mycelium colonization in 23 days and has a 7-day fruiting period.
- It has high protein (30.30%) and amino acids (7.96%), with low fat (1.00%).

## Abstract

To enhance the systematicity and integrity of mushroom resource research by supplementing the mushroom germplasm bank through the collection of wild mushroom specimens, this project focused on a wild mushroom strain collected from Tibet Autonomous Region. The strain was identified and characterized through morphological observation, ITS sequencing, biological trait analysis, and cultivation experiments. The results indicated that the strain belongs to Pleurotus placentodes. The optimal growth conditions for this strain were determined as follows: fructose and sucrose as the preferred carbon source, yeast powder and beef powder as the optimal nitrogen source, pH 5.0, and a temperature of 20 °C. In cultivation experiments, the mycelium fully colonized the substrate in 23 days, primordia formation occurred within 10 d, and the fruiting period lasted 7 d, with an average fresh weight of 39.40 g per fruiting body. Nutritional analysis revealed that P. placentodes has a high protein content (30.30%), high soluble sugar content (6.60%), low fat content (1.00%), and a rich profile of essential amino acids (7.96%). This study successfully isolated, identified, and conducted domestication cultivation and nutritional analysis of a wild P. placentodes, providing scientific references for its potential industrial-scale production.