# Phytofabrication of ZIF-8 Using Mangrove Metabolites for Dual Action Against Drug-Resistant Microbes and Breast Cancer Cells

## TL;DR

This study uses mangrove plant metabolites to create a green nanomaterial that fights drug-resistant microbes and breast cancer cells.

## Contribution

A novel eco-friendly ZIF-8 encapsulated with mangrove metabolites is developed for dual antimicrobial and anticancer action.

## Key findings

- The ZIF-8 material showed broad antimicrobial activity against drug-resistant microorganisms.
- It exhibited significant cytotoxicity against MDA-MB-231 breast cancer cells.
- The nanomaterial was crystalline, rhombic dodecahedron-shaped, and non-agglomerated.

## Abstract

Green nanotechnology offers a sustainable and eco-friendly approach for nanoframework synthesis. The present study intended to synthesize a novel eco-friendly encapsulated Zeolitic Imidazolate Framework-8 (ZIF-8) in a one-pot method using metabolites from the mangrove plant Conocarpus erectus (CE). Gas Chromatography–Mass Spectrometry (GC-MS) analysis of the extract revealed the presence of important bioactive metabolites. The synthesized material was evaluated by UV-Vis spectroscopy, X-ray diffraction (XRD), particle size analysis (PSA), zeta potential measurement, high-resolution transmission electron microscopy (HR-TEM), and Fourier transform infrared (FT-IR) spectroscopy studies. The environment-friendly mangrove metabolites aided by Zeolitic Imidazolate Framework-8 was found to be crystalline, rhombic dodecahedron structured, and size dispersed without agglomeration. The nanomaterial possessed a broad antimicrobial effect on drug-resistant microorganisms, including Candida krusei, Escherichia coli, Streptococcus Sp., Staphylococcus aureus, Enterococcus Sp., Pseudomonas aeruginosa, Klebsiella pneumoniae, C. propicalis, and C. albicans. Further, its cytotoxicity against MDA-MB-231 cells was found to be efficient. The morphological alterations exhibited by the antiproliferative impact on the breast cancer cell line were detected using DAPI and AO/EB staining. Therefore, ZIF-8 encapsulated mangrove metabolites could serve as an effective biomaterial with biomedical properties in the future.