Development of a Collagen–Cerium Oxide Nanohydrogel for Wound Healing: In Vitro and In Vivo Evaluation
Ekaterina Vladimirovna Silina, Natalia Evgenievna Manturova, Victor Ivanovich Sevastianov, Nadezhda Victorovna Perova, Mikhail Petrovich Gladchenko, Alexey Anatolievich Kryukov, Aleksandr Victorovich Ivanov, Victor Tarasovich Dudka, Evgeniya Valerievna Prazdnova
TL;DR
A new collagen-cerium oxide hydrogel was developed and shown to accelerate wound healing in lab and animal tests.
Contribution
A novel collagen–cerium oxide nanohydrogel was developed and validated for wound healing with antioxidant and pro-regenerative effects.
Findings
The hydrogel formulations increased fibroblast proliferation and metabolic activity by 42–50% in vitro.
Nanocomposites reduced DNA damage by up to 45% and provided protection against oxidative radicals.
In vivo tests showed accelerated wound healing with reduced inflammation and improved tissue regeneration.
Abstract
Background: Effective regenerative therapeutics for acute and chronic wounds remain a critical unmet need in biomedicine. Objectives: This study aimed to develop novel collagen–cerium oxide nanoparticle hydrogels designed to enhance cellular metabolism, proliferation, and antioxidant/antimutagenic activity, accelerating wound regeneration in vivo. Methods: Collagen–nanocerium composites were synthesized by combining a collagen extract with cerium oxide nanoparticles at defined concentrations. In vitro assays using human fibroblasts identified two formulations that enhanced proliferation and metabolic activity by 42–50%. FTIR spectroscopy confirmed chemical interactions within the composite matrix. Toxicity, antioxidant, and antigenotoxic effects were evaluated using Escherichia coli MG1655 lux-biosensors to assess their general toxicity, antioxidant and pro-oxidant activities, and…
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Taxonomy
TopicsPeriodontal Regeneration and Treatments · Electrospun Nanofibers in Biomedical Applications · Bone Tissue Engineering Materials