# Adulticidal Activity of the Insect Growth Regulators Methoprene and Cyromazine in House Flies (Musca domestica L.): Evidence from Feeding Trials

## TL;DR

This study shows that insect growth regulators, typically used on larvae, can effectively kill adult house flies when ingested, offering a new approach to pest control.

## Contribution

Demonstrates for the first time that IGRs like methoprene and cyromazine have adulticidal effects when orally administered to house flies.

## Key findings

- Methoprene at 10% concentration achieved 100% mortality in all house fly populations tested.
- Cyromazine induced mortality ranging from 44.28% to 100% across different concentrations.
- IGRs show potential as alternatives to conventional insecticides for sustainable fly control.

## Abstract

House flies (Musca domestica L.) are widespread and significant vectors of various human and animal diseases. In recent years, the development of high levels of resistance to many insecticides has made the control of house flies increasingly difficult. In this study, the acute lethal effects of insect growth regulators (IGRs), usually used to target larva stage, were evaluated by oral administration to adult house flies. The mortality rates observed within 48 h ranged from 70.62% to 100% for all concentrations and populations tested. Our results suggest that IGRs at appropriate concentrations can be effectively used in house flies control strategies and provide a basis for future research in this field.

House flies (Musca domestica L.) are major vectors of numerous pathogens affecting both humans and animals. The global distribution of house flies has been steadily increasing the expansion of human settlements, increased waste production, and the growth of livestock farms established to meet the demand for animal-derived products. Frequent exposure to intensive pesticide applications in agricultural and livestock areas has accelerated the development of insecticide resistance, posing a serious challenge to sustainable control efforts. The widespread and repeated use of conventional chemical insecticides has contributed to rapid resistance evolution in many populations worldwide. In this study, the acute toxic effects of two insect growth regulators (IGRs)—cyromazine and methoprene—commonly used in the larval stages of house flies were evaluated against adult flies. Treatments were applied (3 replicates) orally via 40% sugar-water solutions containing 1%, 5%, and 10% concentrations, and bioassays were conducted on eight distinct house fly populations. The results showed that cyromazine caused average adult mortalities of 76.35%, 81.00%, and 84.50% within 48 h, while methoprene produced 70.62%, 99.37%, and 100% mortality at the same concentrations. At 10%, methoprene achieved 100% mortality across all populations, whereas cyromazine induced mortality ranging from 44.28% to 100%. These findings suggest that IGRs can be effective alternatives to conventional insecticides and can be integrated into IPM/IVM programs to reduce chemical use and delay resistance.