In Vivo and In Vitro Antioxidant Effects of Arthrospira platensis Polysaccharide Component 1 (PAP-1)
Haifeng Yuan, Yuheng Wei, Zhaoyuan He, Xinrui Wang, Xiaoli Yu, Qiuhua Wang, Meiling Yu, Tingjun Hu
TL;DR
This study shows that a purified polysaccharide from Arthrospira platensis has strong antioxidant effects in mice and cells by reducing oxidative stress and boosting antioxidant enzymes.
Contribution
The novel contribution is demonstrating the in vivo and in vitro antioxidant mechanisms of PAP-1 through multiple experimental approaches.
Findings
PAP-1 significantly reduced oxidative stress markers in mice and RAW264.7 cells.
PAP-1 enhanced antioxidant enzyme activities such as SOD, CAT, and GSH-Px.
PAP-1 upregulated key antioxidant genes and proteins including HO-1, NQO1, and GCLM.
Abstract
Arthrospira platensis polysaccharide component 1 (PAP-1), a purified polysaccharide monomer isolated from Arthrospira platensis, exhibits pronounced antioxidant activity. To investigate the in vivo and in vitro regulatory effects of PAP-1 on antioxidant enzyme activities and inflammatory mediators in mice and RAW264.7 cells, the mice were administered PAP-1 by gavage, and the cells were cultured with PAP-1. Subsequently, serum, lung, spleen, and thymus tissues from mice, as well as the cultured RAW264.7 cells, were collected for analysis using RNA sequencing, commercial assay kits, immunohistochemistry, RT-qPCR, and Western blotting. The results demonstrated that PAP-1 significantly reduced the levels of oxidative stress-related indicators (NO, iNOS, MDA, MPO, and XOD), while markedly enhancing the activities of antioxidant enzymes (SOD, CAT, and GSH-Px) (p < 0.05), a trend consistently…
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Taxonomy
TopicsSeaweed-derived Bioactive Compounds · Polysaccharides and Plant Cell Walls · Carbohydrate Chemistry and Synthesis