MIM1 – a selective Mcl-1 protein inhibitor augments the proapoptotic activity of moxifloxacin toward MDA-MB-231 triple-negative breast cancer cells – a preliminary in vitro study

TL;DR

This study shows that combining MIM1 and moxifloxacin increases cell death in a type of aggressive breast cancer, suggesting a new treatment approach.

Contribution

First demonstration of MIM1's strong proapoptotic activity and its synergistic effect with moxifloxacin in TNBC cells.

Findings

MIM1 showed high cytotoxic and proapoptotic potential in MDA-MB-231 cells.

Combining MIM1 with moxifloxacin significantly enhanced apoptosis and mitochondrial disruption.

The synergistic effect suggests a new direction for TNBC treatment strategies.

Abstract

Triple-negative breast cancer (TNBC) is characterized by high invasiveness, high metastatic potential, and poor prognosis. TNBC is not sensitive to endocrine therapy or HER2-targeted treatment, highlighting the need for the development of standardized TNBC treatment regimens. Thus, the development of new TNBC treatment strategies has become an urgent need. MIM1 – BH3 mimetic, which inhibits Mcl-1 antiapoptotic protein, may be an efficacious molecule able to induce apoptosis. Previously, we found that moxifloxacin (MOXI) can modulate the Mcl-1 protein expression. Therefore, in the current study, we assessed the impact of MIM1 and MOXI/MIM1 mixtures on the viability and apoptosis in MDA-MB-231 breast cancer cells.

The viability of cells was assessed by the WST-1 assay. The proapoptotic activity of the tested compounds was determined by cytometric technique.

The results showed that MIM1 exerted high cytotoxic and proapoptotic potential. In the case of two-component models, we have demonstrated that the use of MOXI and MIM1 mixtures resulted in a significant intensification of both cytotoxic and proapoptotic activity – shown as a modulatory effect on mitochondrial membrane potential, cell cycle distribution, or DNA fragmentation, toward MDA-MB-231 cells when compared with MIM1 or MOXI alone.

We have reported, for the first time, the high proapoptotic activity of MIM1 toward MDA-MB-231 cells pointing to the Mcl-1 protein as an important molecular target. Moreover, the observed potential synergistic mode of action (expressed as a significant enhancement of the induction of apoptosis process) detected for MOXI and MIM1 in the two-component model may provide a new direction for further in vitro and in vivo experiments concerning the role of Mcl-1 protein in the treatment of TNBC.

Not applicable.

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