# Early intervention with extracellular vesicles derived from human umbilical cord mesenchymal stem cells enhances survival and functional recovery after spinal cord injury in rats

## TL;DR

Injecting extracellular vesicles from umbilical cord stem cells improves survival and recovery in rats with spinal cord injuries.

## Contribution

Demonstrates that early EV treatment reduces inflammation and promotes recovery in a rat SCI model.

## Key findings

- EV administration improved 30-day survival and locomotor performance from day 7.
- EVs reduced pro-inflammatory cytokines and increased anti-inflammatory and neurotrophic factors.
- EVs accumulated at the injury site and reduced lesion size and glial scar formation.

## Abstract

Spinal cord injury (SCI) is a devastating condition with high mortality and limited treatment options. Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have emerged as promising cell-free therapies due to their immunomodulatory and neuroprotective properties. Here, we evaluated the therapeutic potential of EVs derived from human umbilical cord MSCs in a rat model of SCI.

Adult male Wistar rats were randomized into three groups: control, SCI, and SCI treated with a single intralesional dose of EVs. Locomotor recovery was assessed by the Basso, Beattie & Bresnahan (BBB) score, while survival, neuroinflammation, histological alterations, and biodistribution were systematically analyzed.

EV administration improved 30-day survival, and locomotor performance from day 7, and was associated with sustained reductions in pro-inflammatory cytokines (IL-1β, TNF-α) alongside increased levels of anti-inflammatory cytokines (IL-10) and neurotrophic factors (BDNF). Histological and immunofluorescence analyses showed attenuated microglial activation and astrocytic reactivity, accompanied by reduced lesion size and glial scar formation. In vivo imaging demonstrated accumulation of labeled EVs at the injury site, with peak retention at day 7 post-injection.

Together, these findings demonstrate that early intralesional delivery of MSC-EVs enhances survival, modulates the inflammatory response, and promotes functional recovery after SCI, supporting further translational development of EV-based interventions for SCI.

Diagram depicting the process of using human umbilical cord mesenchymal stem cells to obtain extracellular vesicles (EVs). These EVs are isolated from the mesenchymal stem cell supernatant. The diagram shows the application of EVs to a rodent with a spinal cord injury. Behavioral improvements, such as enhanced locomotor recovery, grip strength, and touch response, are noted. Survival increases while inflammation decreases over days seven to thirty as tested by the BBB and behavior tests.