# OpTiles: an R package for adaptive tiling and methylation variability profiling

## TL;DR

OpTiles is an R package that improves methylation analysis by dynamically defining tiling windows based on CpG distribution and variability.

## Contribution

The package introduces an adaptive tiling method that integrates CpG density and methylation variability for more reliable methylation profiling.

## Key findings

- OpTiles dynamically defines tiling windows based on sequenced CpG distribution.
- It provides a reliability metric by integrating CpG density and intra-region methylation variability.
- The package offers extended functionality for methylation data annotation and interpretation.

## Abstract

OpTiles is an R package that dynamically defines tiling windows based on the distribution of sequenced CpGs, addressing the limitations of traditional fixed-tiling approaches in targeted methylation datasets. By integrating CpG density with intra-region methylation variability, it provides a reliability metric and extended functionality for annotating, prioritizing, and interpreting complex methylation data.

OpTiles is implemented in R and source code is freely available at https://github.com/fhaive/OpTiles. Data are available on Zenodo at https://doi.org/10.5281/zenodo.16961292.