# Overexpression of the translocon accessory protein YajC alleviates toxicity of the endogenous pore-forming toxin LdrA in Escherichia coli

## TL;DR

This study shows that overexpressing YajC, a translocon accessory protein, can reduce the toxicity of the LdrA toxin in E. coli.

## Contribution

The study reveals a novel role for YajC in counteracting pore-forming toxins like LdrA, beyond its known translocation function.

## Key findings

- Overexpression of YajC alleviates LdrA toxicity without affecting its localization or stability.
- YajC's suppressive activity is specific to the Ldr toxin family, including LdrA and LdrD.
- YajC requires both transmembrane and cytoplasmic domains to recognize LdrA.

## Abstract

Almost all bacterial species possess toxin-antitoxin (TA) systems. In Escherichia coli, LdrA is a small membrane protein that functions as a pore-forming toxin. Expression of LdrA results in transient growth arrest, from which cells subsequently recover; however, the underlying recovery mechanism remained unknown. In the present study, we investigated the molecular basis for this recovery. Using a genetic screen, we identified YajC, an accessory protein of the SecDF-YajC translocon, as a key factor in counteracting of LdrA toxicity. We demonstrate that overexpression of YajC effectively alleviates LdrA toxicity without affecting its membrane localization or stability. This suppressive activity was specific for the Ldr toxin family, including LdrA and its homolog LdrD. Furthermore, our results indicate that YajC recognition of LdrA requires both its transmembrane and cytoplasmic domains. These findings reveal a novel function for YajC in counteracting a membrane-damaging toxin, distinct from its known role in the Sec-dependent protein translocation pathway.