# Activity-Dependent Increases in Quantal Size at the Drosophila NMJ

## TL;DR

This study shows that increased nerve activity at fruit fly neuromuscular junctions leads to larger synaptic signals, likely due to calcium-dependent processes.

## Contribution

The paper demonstrates that activity-dependent increases in quantal size are a general property of Drosophila NMJ synapses.

## Key findings

- Prolonged or brief nerve stimulation increases quantal size at all four muscle fibers studied.
- Activity-dependent increases in quantal size and frequency depend on postsynaptic Ca2+.
- Glutamate-evoked responses are potentiated by Ca2+ and require calmodulin or CaMKII activation.

## Abstract

We examined whether an increase in synaptic activity resulted in an increase in quantal size at the neuromuscular junction (NMJ) of third-instar Drosophila larvae. Spontaneous miniature excitatory postsynaptic currents (mEPSCs) or miniature excitatory postsynaptic potentials (mEPSPs) were recorded before and after nerve stimulation. We found that prolonged (60 s) or brief (1.25 s) nerve stimulation produced an increase in quantal size; this appears to be a general property of these synapses since it was seen at all four muscle fibers (MFs) used in this study. The effect was examined along Is and Ib terminals by expressing GCaMP in the MF membrane and examining postsynaptic Ca2+ signals produced by spontaneous transmitter release. The activity-dependent increase in quantal size occurred at both Is and Ib terminals, and the increase in frequency and amplitude of quantal events at individual synaptic boutons was correlated. Both the increase in quantal size and frequency were found to be dependent upon an increase in postsynaptic Ca2+, based on studies in which MFs were preinjected with the Ca2+ chelator BAPTA (1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid). To examine the effect of postsynaptic activity on glutamate sensitivity, we iontophoresed glutamate pulses at the NMJ and recorded the glutamate-evoked excitatory postsynaptic potentials (gEPSPs). Trains of glutamate pulses produced an increase in gEPSP amplitude; this potentiation was not seen when Ca2+ was eliminated from the bath or after inhibiting calmodulin or CaMKII. The activity-dependent increase in quantal size may result from an increase in postsynaptic sensitivity due to activation of CaMKII.