# Cloned Pig Fetuses Have a High Placental Lysophosphatidylcholine Level That Inhibits Trophoblast Cell Activity

## TL;DR

Cloned pig fetuses have high levels of a lipid called lysophosphatidylcholine, which harms placental development and reduces cloning success.

## Contribution

This study identifies elevated lysophosphatidylcholine in cloned pig placentas as a novel factor impairing trophoblast cell activity and placental development.

## Key findings

- SCNT placentas show impaired lipid metabolism and elevated lysophosphatidylcholine (LPC) levels.
- LPC inhibits the proliferation and migration of porcine trophoblast cells.
- High LPC levels are linked to abnormal placental development in cloned pig fetuses.

## Abstract

Somatic cell nuclear transfer (SCNT) or cloning technology is widely used in agriculture and biomedicine. However, the application of this technology is limited by the low developmental competence of cloned embryos or fetuses, which frequently exhibit abnormal development of trophoblast cells or placentas. The purpose of this study was to investigate the possible causes of the erroneous placental development of SCNT-derived pig fetuses. The placental transcriptomic and lipidomic profiles were compared between 30-day-old SCNT- and artificial insemination (AI)-produced pig fetuses. Differentially expressed lipid metabolites between two groups of placentas were selected to test their effects on porcine trophoblast cell activity. The results showed that SCNT placentas exhibit impaired lipid metabolism and function. The level of a metabolite, lysophosphatidylcholine (LPC), in the glycerophospholipid metabolism pathway was substantially increased in SCNT placentas, compared with AI placentas. The elevation in LPC content may lead to impaired placental development in cloned pig fetuses, as LPC inhibited the proliferation and migration of porcine trophoblast cells. This study discovers a main cause of erroneous development of cloned pig fetuses, which will be beneficial for understanding the regulation of SCNT embryo development, as well as developing new methods to improve the efficiency of pig cloning.