# Evaluation of the Effects of Dorema aucheri (Bilhar) Hydroalcoholic Extract on Wound Healing of Streptozotocin-Induced Diabetic Rats: A Stereological Study

## TL;DR

This study shows that a 5% extract of Dorema aucheri helps heal diabetic wounds in rats faster and better than other concentrations.

## Contribution

The study introduces the effectiveness of 5% Dorema aucheri extract in improving diabetic wound healing in rats.

## Key findings

- 5% D. aucheri extract improved healing speed, re-epithelialization, and collagen deposition in diabetic wounds.
- 10% D. aucheri extract reduced fibroblast and collagen density compared to other groups.
- Wound healing was measured using stereological and photographic methods over 14 days.

## Abstract

One of the most common complications of diabetes is impaired healing of diabetic wounds. Given the antidiabetic and antimicrobial effects of the Dorema aucheri plant, the present study was conducted to determine the impact of the alcoholic extract of this plant on the healing of full-thickness diabetic wounds in male rats.

In this experimental study, 48 male rats were randomly divided into 6 groups: control wound, diabetic wound, diabetic wound with carboxymethylcellulose base gel, and diabetic wounds with the combination of D. aucheri extract with percentages of 2%, 5%, and 10%. The wound in all groups was the full thickness of the skin. The drugs were administered topically, once daily, for 14 days. Also, to measure the percentage of wound healing, we took photographs of the samples on Days 0, 3, 7, and 14. Finally, skin samples were taken from the wound repair site for stereological evaluation.

In comparison to the other groups, the group treated with 5% D. aucheri extract showed a better healing speed, volume of re-epithelialization, density of collagen deposition, and neovascularization (p ≤ 0.05). The fibroblast number density, collagen density, and hair follicle volume densities were all lower in the 10% D. aucheri-treated group than in the other groups (p ≤ 0.001).

The hydroalcoholic extract of the D. aucheri plant, particularly at a concentration of 5%, has beneficial effects on the healing process of diabetic wounds.