# Nitric oxide attenuates PI4P accumulation at the ER membrane to inhibit encephalomyocarditis virus replication selectively in β-cells

## TL;DR

Nitric oxide prevents a type of virus from replicating in specific cells by reducing a key molecule in their membranes.

## Contribution

Nitric oxide selectively inhibits EMCV replication in β-cells by reducing ER membrane PI4P accumulation.

## Key findings

- NO inhibits EMCV replication by reducing phosphatidylinositol-4-phosphate at the ER membrane.
- This inhibition prevents viral replication complex formation in β-cells.
- The effect is selective for β-cells due to ATP depletion.

## Abstract

Viral infection, particularly by members of the picornavirus family, has been associated with autoimmune diabetes (type 1 diabetes mellitus) onset. The encephalomyocarditis virus (EMCV) is a mouse-tropic member of the picornavirus family that stimulates innate immune activation, leading to the production of cytokines. In response to cytokines, β-cells express inducible nitric oxide (NO) synthase and produce low micromolar levels of the free radical, NO. We have previously shown that, because of its inhibitory action on mitochondrial oxidation and depletion of cellular ATP, NO selectively attenuates EMCV replication in, and lysis of, β-cells. In this study, we show that one mechanism by which NO inhibits EMCV replication is by attenuating the accumulation of phosphatidylinositol-4-phosphate at the endoplasmic reticulum membrane. As a result, viral replication complex formation is prohibited, and viral replication is effectively prevented. In agreement with previous studies, we show that these observations are selective for β-cells and because of a loss of cellular ATP.