# Mild hyperbaric oxygen does not attenuate mitochondrial decrease induced by detraining in mice

## TL;DR

Mild hyperbaric oxygen does not prevent muscle and mitochondrial loss after training cessation in mice.

## Contribution

This study shows MHO fails to counteract detraining effects on muscle and mitochondrial adaptations in mice.

## Key findings

- Detraining reduced soleus muscle weight and mitochondrial enzyme activity in mice.
- MHO did not prevent the loss of antioxidant proteins or mitochondrial function after detraining.

## Abstract

Mild hyperbaric oxygen (MHO) attenuates the muscle atrophy caused by muscle disuse. Training cessation results in the partial or complete loss of training-induced adaptations, including mitochondrial enzyme activities. The present study aimed to determine whether exposure to MHO after running training attenuated negative adaptation induced by detraining. We allocated eight-week-old mice into training (Tr), detraining after the training period (DeTr), and detraining + mild hyperbaric oxygen (DeTr + MHO, 1.3 atm absolute with 38 % oxygen) groups. Mice underwent voluntary wheel running for four weeks, followed by a two-week detraining period under normal or MHO conditions. The soleus muscle weight (mg/g BW) decreased by approximately 30 % in the DeTr and DeTr + MHO groups compared to the Tr group (P < 0.001 and 0.01, respectively). Citrate synthase (CS) activity, the expression of mitochondrial complex IV, antioxidant-related proteins, catalase, and heme oxygenase 1 (HO-1), decreased in the DeTr and DeTr + MHO group compared to the Tr group (P < 0.05). In summary, MHO did not attenuate the detraining-induced decrease in soleus muscle weight relative to body weight, mitochondrial enzyme activity, protein, or antioxidant protein expression level in the plantaris muscle after a four-week training period using voluntary wheel running in mice.

•Detraining reduced soleus weight and plantaris mitochondrial enzyme activity.•MHO did not prevent the decrease in soleus weight after detraining.•MHO failed to prevent mitochondrial and antioxidant protein loss.

Detraining reduced soleus weight and plantaris mitochondrial enzyme activity.

MHO did not prevent the decrease in soleus weight after detraining.

MHO failed to prevent mitochondrial and antioxidant protein loss.