# Historical Insights Into the Purification of Polyphenol Oxidase From Plants

## TL;DR

This paper reviews the history and methods of purifying polyphenol oxidase from plants over the past 50 years.

## Contribution

The paper provides a comprehensive overview and comparison of PPO purification techniques and their biochemical relevance.

## Key findings

- Purification methods have evolved to achieve partially purified or pure polyphenol oxidase.
- Biochemical properties of PPO are crucial for its biotechnological applications.
- Structural similarities among PPO from different species were identified through amino acid sequence and tertiary structure comparisons.

## Abstract

Over the past 50 years, different methods have been developed to purify polyphenol oxidase (PPO), a key enzyme involved in the oxidation of phenolic compounds in plants. This review presents a comprehensive summary of the advances in purification techniques, highlighting the evolution of the methods used over the years to achieve partially purified or pure enzymes, while also discussing their advantages and limitations. Furthermore, this review examines the biochemical properties of PPO that are most significant for its potential biotechnological applications. A comparison of the amino acid sequences and tertiary structures of crystallized PPO is also included, enabling the identification of structural similarities among PPO from different species. Finally, this review has been written as a guide for decision‐making regarding the selection of an appropriate PPO purification strategy, taking into account the required level of purification and the enzyme's source.

Overview of purification methods for polyphenol oxidase. Advancements in achieving partial or homogeneous purification. Key biochemical properties relevant to polyphenol oxidase.