# A CRISPR/Cas9-induced restoration of bioluminescence reporter system for single-cell gene expression analysis in plants

## TL;DR

Researchers developed a CRISPR-based system to restore bioluminescence in plants, enabling detailed single-cell gene expression analysis.

## Contribution

A novel CRISPR/Cas9-based system (CiRBS) was developed to restore bioluminescence in transgenic Arabidopsis for single-cell gene expression analysis.

## Key findings

- CiRBS successfully restored bioluminescence in 7.2% of CRISPR/Cas9-transfected cells.
- Recombination events via indels were mostly complete within 24 hours of CRISPR/Cas9 induction.
- 94% of bioluminescence-restored cells had only one chromosome with optimal recombination.

## Abstract

Bioluminescence monitoring techniques have greatly contributed to revealing a variety of biological regulatory systems in living organisms, including circadian clocks. In plant science, these techniques are applied to long-term quantitative analyses of gene expression behavior. Transient transfection with a luciferase reporter using the particle bombardment method has been used for bioluminescence observations at the single-cell level. This allows for capturing heterogeneity and temporal fluctuations in cellular gene expression, although bioluminescence could fluctuate according to variation in physiological factors associated with the luciferase reaction. We developed a novel CRISPR/Cas9-induced restoration of bioluminescence reporter system, CiRBS, to monitor cellular bioluminescence from a reporter gene in the genome of transgenic Arabidopsis. In this method, the enzymatic activity of an inactive luciferase mutant, LUC40Ins26bp, which has a 26-bp insertion at the 40th codon, was restored by introducing an indel at the insertion site using CRISPR/Cas9. We succeeded in long-term monitoring of the cellular bioluminescence of Arabidopsis plants expressing LUC40Ins26bp, which was restored by transient transfection with CRISPR/Cas9-inducible constructs using particle bombardment. Recombination events via indels were mostly complete within 24 h of CRISPR/Cas9 induction, and 7.2% of CRISPR/Cas9-transfected cells restored bioluminescence. It was estimated that 94% of the bioluminescence-restored cells carried only one chromosome having the optimal recombination construction. Thus, CiRBS allows for reliable single-cell gene expression analysis of cell-to-cell heterogeneity and temporal fluctuations from a single locus.

The online version contains supplementary material available at 10.1038/s41598-025-25062-9.