# Addressing fatty tissue in quantitative susceptibility mapping of human knee cartilage

## TL;DR

This study examines how excluding fatty tissue affects susceptibility contrast in knee cartilage imaging, finding that including fatty tissue improves contrast between cartilage layers.

## Contribution

The study introduces a comparison of masking strategies in QSM to assess the impact of excluding fatty tissue on cartilage susceptibility contrast.

## Key findings

- Excluding fatty tissue or bone marrow reduced the susceptibility contrast between cartilage layers.
- All tested methods produced adequate image quality for knee cartilage QSM.
- Inclusion of fatty tissue may be preferable if chemical shift contributions are addressed.

## Abstract

To evaluate the effects of excluding fatty tissue in QSM of human knee cartilage.

Gradient echo images from 18 knee-healthy volunteers were acquired, from which chemical shift corrected field perturbation maps were calculated. Based on these, QSM maps were reconstructed using morphology enabled dipole inversion and one of three masking alternatives: (1) excluding no tissue, (2) excluding bone marrow, and (3) excluding all fatty tissues. The slope of a linear regression [ppm/%] between susceptibility values and the relative distance from the bone surfaces was used as a measurement of contrast between cartilage layers. The average differences in slopes between methods are reported with 95% confidence intervals.

The expected susceptibility differences between cartilage layers from literature were observed for all tested reconstruction techniques. However, smaller slopes (average difference (confidence interval)) were detected when either all fatty tissue (− 0.090 (− 0.121, − 0.059) ppm/%) or bone marrow (− 0.088 (− 0.121, − 0.055) ppm/%) was excluded from reconstruction.

All tested methods result in adequate image quality in QSM of knee cartilage. However, exclusion of fatty tissue decreased the susceptibility contrast between cartilage layers. Assuming that phase contributions from chemical shift are addressed, inclusion of fatty tissue may be preferable.