# A dedicated phantom for exploring the interplay of fat and paramagnetic substances in quantitative susceptibility mapping

## TL;DR

Researchers created a phantom to study how fat and iron affect MRI measurements in the liver, helping improve imaging accuracy.

## Contribution

A new phantom was developed to explore the effects of fat and paramagnetic substances on quantitative susceptibility mapping in liver-like conditions.

## Key findings

- The phantom's magnetic properties closely resemble those of liver tissue.
- Fat correction in susceptibility maps reduced artifacts and increased susceptibility values.
- Stable measurements were achieved across multiple scans and over 14 months.

## Abstract

Accurate quantitative tissue characterization in organs with considerable fat content, like the liver, requires thorough understanding of fat’s influence on the MR signal. To continue the investigations into the use of quantitative susceptibility mapping (QSM) in abdominal regions, we present a dedicated phantom that replicates liver-like conditions in terms of effective transverse relaxation rates (R2*) and proton density fat fractions.

The spherical agar phantom consists of nine smaller spheres (diameter: 3 cm) doped with a paramagnetic substance (iron nanoparticles or manganese chloride) and fat (peanut oil), embedded in a large agar sphere (diameter: 14 cm), ensuring no barriers exist between the enclosed spheres and their surrounding medium. Concentrations were selected to represent both healthy and pathologic conditions. 3T MRI measurements for relaxometry, fat–water imaging, and QSM were conducted with the head coil and for 1H-spectroscopy with the knee coil at three time points, including a scan–rescan assessment and a follow-up measurement 14 months later.

The phantoms’ relaxation and magnetic properties are in similar range as reported for liver tissue. Substantial alterations in local field and susceptilibty maps were observed in regions with elevated fat and iron content, where fat correction of the local field via chemical shift-encoded reconstruction effectively reduced streaking artifacts in susceptibility maps and substantially increased susceptibility values. Linear regression analysis revealed a consistent linear relationship between R2* and magnetic susceptibility, as well as iron concentration and magnetic susceptibility. The relaxation, fat, and susceptibility measurements remained stable across scan–rescan assessment and long-term follow-up.

We developed a versatile phantom to study fat–iron interactions in abdominal imaging, facilitating the optimization and comparison of susceptibility processing methods in future research.

The online version contains supplementary material available at 10.1007/s10334-025-01261-3.