# Characterization of the complete mitochondrial genome data of Asiatic Toad, Bufo gargarizans (Cantor, 1842) (Neobatrachia: Bufonidae) from Mianyang, China

## TL;DR

This paper reports the full mitochondrial genome of the Asiatic toad from China, providing insights into its genetic structure and evolutionary relationships.

## Contribution

The study provides the first complete mitochondrial genome sequence of Bufo gargarizans from Mianyang, China, along with its phylogenetic analysis.

## Key findings

- The mitochondrial genome is 17,431 base pairs long with typical gene content including 13 protein-coding genes.
- The genome has a high AT content (57.17%) and forms a monophyletic group with other Bufonidae species.
- The genome data is available in GenBank under accession number PV083742.

## Abstract

A common amphibian in East Asia, the Bufo gargarizans is significant for research on ecology, conservation biology, and evolution. Thus, the B. gargarizans mitochondrial genome was sequenced and subjected to a methodical analysis. The sequence was 17,431 base pairs long, with 13 protein-coding genes (PCG), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and a D-loop control region. The nucleotide base composition of the mitochondrial genome was skewed toward AT content (57.17%), including adenine (28.80%), thymine (28.37%), cytosine (27.40%), and guanine (15.43%). For phylogenetic analysis, Bayesian inference (BI) techniques were used to build a phylogenetic tree from the mitochondrial genomes of 27 other species and the B. gargarizans. The findings demonstrated that species of the Bufonidae family formed a monophyletic group with the B. gargarizans. The mitochondrial genome of B. gargarizans will serve as an invaluable asset for future investigations into the evolution, taxonomy, and genetic preservation strategies of this species. Mitochondria genomic data can be found in GenBank under accession number PV083742.