# Simple gene knockout by single gene-directed multiplex CRISPR-Cpf1 **Authors:** Yeon-Ju Jeong, Gyeong-Nam Kim, Jeongin Cho, Young Hoon Sung PMC · DOI: 10.1016/j.gendis.2025.101646 · Genes & Diseases · 2025-04-21 ## Full-text entities - **Genes:** EI24 (EI24 autophagy associated transmembrane protein) [NCBI Gene 9538] {aka EPG4, PIG8, TP53I8}, CRIPTOP5 (CRIPTO pseudogene 5) [NCBI Gene 22814] {aka CR-5, CRIPTO-5, TDGF1P5, TDGF5}, MAP1LC3A (microtubule associated protein 1 light chain 3 alpha) [NCBI Gene 84557] {aka ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3}, POTEF (POTE ankyrin domain family member F) [NCBI Gene 728378] {aka A26C1B, POTE2alpha, POTEACTIN}, CRIPTOP4 (CRIPTO pseudogene 4) [NCBI Gene 22815] {aka CR-4, CRIPTO-4, TDGF1P4, TDGF4}, CRIPTO3 (cripto, EGF-CFC family member 3) [NCBI Gene 6998] {aka CR-3, CRIPTO-3, TDGF1, TDGF1P3, TDGF2, TDGF3}, CR1 (complement C3b/C4b receptor 1 (Knops blood group)) [NCBI Gene 1378] {aka C3BR, C4BR, CD35, KN}, CRIPTOP6 (CRIPTO pseudogene 6) [NCBI Gene 22813] {aka CR-6, CRIPTO-6, TDGF1P6, TDGF6}, ATG5 (autophagy related 5) [NCBI Gene 9474] {aka APG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5} - **Diseases:** cancer (MESH:D009369), hepatocellular carcinoma (MESH:D006528) - **Chemicals:** BSD (MESH:C004500), phosphatidylethanolamine (MESH:C483858), 4CR (-), 4',6-diamidino-2-phenylindole (MESH:C007293) - **Species:** Acidaminococcus sp. (species) [taxon 1872103], teschovirus A1 (no rank) [taxon 85506], Homo sapiens (human, species) [taxon 9606] - **Cell lines:** BJ — Homo sapiens (Human), Telomerase immortalized cell line (CVCL_6573), SNU475 — Homo sapiens (Human), Adult hepatocellular carcinoma, Cancer cell line (CVCL_0497) ## Full text _Full body text omitted from this summary view._ Fetch the complete paper as Markdown: https://tomesphere.com/paper/PMC12637181/full.md ## Figures 1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12637181/full.md ## References 5 references — full list in the complete paper: https://tomesphere.com/paper/PMC12637181/full.md --- Source: https://tomesphere.com/paper/PMC12637181